DYNAMIC: Advanced Antibacterial Biomaterials to Enhance Bone Regeneration

DYNAMIC focuses on three main objectives:

1. Development of smart molecules: The design of linkers (molecular connectors) that activate only in the presence of a bacterial infection, releasing antibacterial peptides in a controlled and temporary manner. These molecules will detect specific signals from the infection environment, such as certain enzymes or changes in pH.
2. Creation of advanced biomaterials: The combination of these smart molecules (linkers + antibacterial peptides) with peptides that promote cellular adhesion or polydopamine nanoparticles to produce multifunctional biomaterials (titanium implants and hydrogels). These combinations will have unique properties to help cells adhere and dynamically fight bacteria.
3. Advanced interaction studies: Using these materials as advanced platforms to study, in real time, the interactions between cells and bacteria, aiming to develop new biomaterials that improve bone regeneration in complex infected environments.

To achieve this, firstly, degradable linkers will be synthesised under specific conditions, such as the acidic pH typical of bacterial infections or the action of proteases, and an antibacterial peptide (LF1-11) will be added. At the same time, these linkers will be combined with cell adhesion peptides on peptide platforms or with polydopamine nanoparticles to enhance tissue fixation.

Secondly, titanium surfaces and hydrogels will be functionalised with multifunctional coatings to create dynamic materials with osteointegrative and antibacterial properties.

Thirdly, their effectiveness will be evaluated by testing these materials with stem cells and various types of bacteria to analyse their performance under controlled conditions. Finally, the best combinations will be tested in a proximal tibia bone infection repair model to assess the ability of the biomaterials to induce bone formation in vivo under infection conditions.

DYNAMIC proposes an innovative and unprecedented approach to developing multifunctional biomaterials that stimulate bone growth and deliver antibacterial agents in a controlled and temporary manner in the context of infection. This strategy offers a novel solution for bone tissue regeneration and represents a substantial advancement in the field.

Budget and Funding

The project will run for 3 years (September 2024–August 2027) with a budget of €200,000, funded by the 2023 Knowledge Generation Projects programme (PID2023-148538OB-I00) (Spanish State Research Agency).